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be ‘lost’ because not all the fibres could sufficiently deform to adhere to the underlying gold surface. Thus the morphology of substrates before and after etching should not totally be the same. Image J was used for image analysis and quantification of data.
　　Aim 3 is to study the impact of substrate morphology, fibre dimension and density on cell adhesion
　　Due to the time limitation, it is not permitted to do all the cell assays on the patterned substrates. One hypothesis of the cell assay is that as the density changes, the cell shape will change accordingly. The impact of fibre diameter on focal adhesion formation was studied. The effect of fiber density on the number of cells adhering to the substrate is another parameter to control.

Chapter 3 Electrospinning .....................................20
3.1 Introduction .....................................20
3.2 Experimental section..................................... 20
3.3 Results and discussion..................................... 21
3.4 Conclusion .....................................24
Chapter 4 Etching..................................... 25
4.1 Introduction..................................... 25
4.2 Experimental section .....................................27
4.3 Results and discussion..................................... 28
4.4 Conclusion .....................................33
Chapter 5 Fibronectin staining..................................... 34
5.1 Introduction..................................... 34
5.2 Experimental section..................................... 35
5.3 Results and discussion..................................... 36
5.4 Conclusion..................................... 38

Chapter 6 Conclusions and Future Considerations结论和未来的考虑
　　在这个项目的总结中，一个新的微图案化的方法是基于静电纺丝和湿法腐蚀之上In summary of this project, a novel method of micro-patterning methods has been developed based on electrospinning and wet etching. 4 sizes of the level scale of 150nm, 300nm, 600nm and 900nm PMMA polymer (MW=350,000) were deposited on the gold coated glass/silicon sides. After etching by KI/I2 solution, the diameter of fibrillar gold left on the substrate has a significant increase compared to the original fibre dimension: from 166.2nm to 238.7nm, 321.7nm to 457.9nm, 597.5nm to 660.1nm, 877.6nm to 1203.4nm. The most possiblity for this change is after annealing the fibres change size and become flat, which may expand the contact aeras with the substrates. For each sizes of patterns, we got two densities: 12.7% for 900nm thin patterns, 24% for 900nm thick patterns, 27.2% for 600nm thin patterns, 43.7% for 600nm thick patterns, 22% for 300nm thin patterns, 39.6% for 300nm thick patterns, 11.1% for 150nm thin patterns 23.4% for 150nm thick patterns. All the fabricated patterns were examined by fibronectin staining. Comparing the fluorescence microscope images after staining with the SEM images after etching, there is little alteration occurs between the density and human cell could adhered on the patterns and displayed specific shape. All the data and results prove this approach is a successful micropatterning method. Compared with conventional patterning approaches, this method is more economic and alterable since it does not need any stable patterns and mask. Furthermore, mor本论文由英语论文网提供整理，提供论文代写，英语论文代写，代写论文，代写英语论文，代写留学生论文，代写英文论文，留学生论文代写相关核心关键词搜索。