英语论文网

留学生硕士论文 英国论文 日语论文 澳洲论文 Turnitin剽窃检测 英语论文发表 留学中国 欧美文学特区 论文寄售中心 论文翻译中心 我要定制

Bussiness ManagementMBAstrategyHuman ResourceMarketingHospitalityE-commerceInternational Tradingproject managementmedia managementLogisticsFinanceAccountingadvertisingLawBusiness LawEducationEconomicsBusiness Reportbusiness planresearch proposal

英语论文题目英语教学英语论文商务英语英语论文格式商务英语翻译广告英语商务英语商务英语教学英语翻译论文英美文学英语语言学文化交流中西方文化差异英语论文范文英语论文开题报告初中英语教学英语论文文献综述英语论文参考文献

ResumeRecommendation LetterMotivation LetterPSapplication letterMBA essayBusiness Letteradmission letter Offer letter

澳大利亚论文英国论文加拿大论文芬兰论文瑞典论文澳洲论文新西兰论文法国论文香港论文挪威论文美国论文泰国论文马来西亚论文台湾论文新加坡论文荷兰论文南非论文西班牙论文爱尔兰论文

小学英语教学初中英语教学英语语法高中英语教学大学英语教学听力口语英语阅读英语词汇学英语素质教育英语教育毕业英语教学法

英语论文开题报告英语毕业论文写作指导英语论文写作笔记handbook英语论文提纲英语论文参考文献英语论文文献综述Research Proposal代写留学论文代写留学作业代写Essay论文英语摘要英语论文任务书英语论文格式专业名词turnitin抄袭检查

temcet听力雅思考试托福考试GMATGRE职称英语理工卫生职称英语综合职称英语职称英语

经贸英语论文题目旅游英语论文题目大学英语论文题目中学英语论文题目小学英语论文题目英语文学论文题目英语教学论文题目英语语言学论文题目委婉语论文题目商务英语论文题目最新英语论文题目英语翻译论文题目英语跨文化论文题目

日本文学日本语言学商务日语日本历史日本经济怎样写日语论文日语论文写作格式日语教学日本社会文化日语开题报告日语论文选题

职称英语理工完形填空历年试题模拟试题补全短文概括大意词汇指导阅读理解例题习题卫生职称英语词汇指导完形填空概括大意历年试题阅读理解补全短文模拟试题例题习题综合职称英语完形填空历年试题模拟试题例题习题词汇指导阅读理解补全短文概括大意

商务英语翻译论文广告英语商务英语商务英语教学

无忧论文网

联系方式

美国医学专业作业-Plasma IKK NF-kB

论文作者:www.51lunwen.org论文属性:作业 Assignment登出时间:2014-06-01编辑:lzm点击率:10476

论文字数:2840论文编号:org201406012014576248语种:英语 English地区:中国价格:免费论文

关键词:美国医学专业Plasma IKK NF-kB血浆游离脂肪酸free plasma fatty acidsreactive oxygen species

摘要:This study examines the effect of prolonged exposure to FFA on the NF-kB activated inflammatory pathway. In vivo, infusion of oleate impaired insulin secretion as measured by c-peptide, while olive oil did not impair insulin secretion but impaired insulin sensitivity.

Abstract:
 
An important mechanism of pathogenesis of type 2 diabetes in obese individual is the elevation of free plasma fatty acids (FFA) and generation of reactive oxygen species (ROS). The objective of the current study is to determine whether the IKK/NF-kB inflammatory pathway also plays a role in liptoxicity. The IKK inhibitor salicylate was used in this study. Study was conducted in vivo using hyperglycemic clamp, ex-vivo with isolated islets, and in vitro.
 
Female Wistar Rats were infused with either a)oleate, b)olive oil+heparin, c)oleate+salicylate, d)olive oil + salicylate, e)salicylate, or f)saline for 48hrs. A two-step hyperglycemic clamp was followed or the islets were isolated. Islets were assayed for insulin and c-peptide.
 
Oleate and olive oil reduce GINF during the two-step hyperglycemic clamp. Oleate impaired insulin secretion when c-peptide was measured. This was prevented by the co-infusion of salicylate. Olive oil decreased insulin sensitivity when the sensitivity index was calculated. The effect of olive oil on insulin sensitivity is prevented by the co-infusion of salicylate. Ex-vivo and in vitro data showed similar result. The data here presented demonstrates the role of IKK/NF-kB in FFA induced liptoxicity and its prevention by salicylate.
 
Introduction:
 
Diabetes mellitus 2 is characterized by a combination of glucotoxicity and lipotoxicity. Evidences supporting glucotoxicity mediated decrease in β-cell function are well documented. However the mechanisms of free fatty acid (FFA) induced liptoxicity is not fully elucidated. FFA is needed acutely for glucose induced insulin secretion (GSIS). However, when chronically exposed to high levels of FFA, β-cell function decreases due to increased insulin resistance or impaired insulin secretion. In vitro results have shown that increased levels of FFA impair GSIS, in vivo results are more controversial with different groups presenting conflict results. Despite this mounting evidence suggest increased levels of FFA in obesity leads to insulin resistance. In vivo a decrease in insulin resistance should be accompanied by a corresponding increase in insulin secretion; this is not seen in individuals pre-disposed to type-2 diabetes, suggesting that other pathway may also be involved. One theory proposes that the release of adipose tissue mass is responsible for intracellular alternations that reduce insulin signaling. Adipose tissues are known to release cytokines such as TNF-α, which causes insulin resistance by serine phosphorylation of the insulin receptor and its substrate (IRS-1). Recently it has been shown that another pathway that elevated FFA may cause β-cell dysfunction is through the generation of reactive oxygen species (ROS).
 
Previous studies have demonstrated that islets cells are especially susceptible to oxidative stress due to inherent low anti-oxidant defense. Proposed pathways of liptoxicity such as activation of JNK, PKC, or IKK/NF-kB pathway are often either upstream or downstream of oxidative stress. Studies conducted in this lab have demonstrated that antioxidants such as taurine, N-Acetylcysteine (NAC), and TEMPOL are able to prevent β-cell dysfunction in rats with elevated FFA levels. Cytosolic superoxide is found to be increased in oleate infused rat while mitochondrial superoxide was not increased.论文英语论文网提供整理,提供论文代写英语论文代写代写论文代写英语论文代写留学生论文代写英文论文留学生论文代写相关核心关键词搜索。

共 1/6 页首页上一页123456下一页尾页

英国英国 澳大利亚澳大利亚 美国美国 加拿大加拿大 新西兰新西兰 新加坡新加坡 香港香港 日本日本 韩国韩国 法国法国 德国德国 爱尔兰爱尔兰 瑞士瑞士 荷兰荷兰 俄罗斯俄罗斯 西班牙西班牙 马来西亚马来西亚 南非南非