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论文作者:www.51lunwen.org论文属性:硕士毕业论文 dissertation登出时间:2014-08-01编辑:felicia点击率:16847
论文字数:8677论文编号:org201407281357193486语种:英语 English地区:澳大利亚价格:免费论文
关键词:Plant Pollinator Interactions花卉色彩多样性澳大利亚floral colour diversity生物学
摘要:本文是一篇生物学留学论文。由于地质经历了漫长的发展阶段,澳大利亚大陆与其他大陆长期隔离,因此动植物物种有着显著的差别。在花卉色彩多样性的进化过程中,对这方面的研究极少。本文通过分析植物间花粉的传播,进一步分析澳大利亚花卉色彩的多样性。
Innate colour preference testing
To test the innate colour preferences of naive bees, I performed simultaneous choice experiments with flower-naive bees using artificial flowers that simulated the floral colours of natural flowers. The aluminum rewarding disks were replaced by the ten unrewarding, coloured artificial disks in the original flight arena. Artificial flower stimuli were cut in a circle (70 mm diameter) from standardized colour papers of the HKS-N-series (Hostmann-Steinberg K+E Druckfarben, H. Schmincke & Co., Germany). In each experiment the same set of ten test colours (1N - pale yellow, 3N - saturated yellow, 21N - light pink, 32N - pink, 33N - purple, 50N - blue, 68N - green, 82N - brown, 92N - grey, back of 92N - white) were used. These colours were chosen as they have been used in innate colour experiments with other hymenopterans (Giurfa, Núñez et al. 1995; Kelber 1997; Gumbert 2000), and the colours are also widely used in other bee colour experiments (Giurfa, Vorobyev et al. 1996).
The coloured paper disks were placed on vertical plastic cylinders (diameter = 15 mm; height = 50 mm), to raise them above the floor of the flight arena. The gate was shut in the arena to ensure the bees used in each trial were separated from the next trial. The number of landings and approaches to the stimuli were recorded for one hour. Approximately 200 bees were used for each trial. The spatial positions of the artificial flowers were pseudo randomised in a counter balance fashion every 15 minutes. After each trial, the colour disks were aired and wiped with a paper tissue to remove possible scent marks, which are known to affect experiments with honeybees (Schmitt and Bertsch 1990; Giurfa and Núñez 1992). I conducted each subsequent trial after removing the used bees from the system, to ensure that the bees in the next trial were replaced with naive foragers.
It is known that perception of colour can be influenced by background colour (Lunau, Wacht et al. 1996). Therefore, I also tested colour choices on other background colours of grey and black. The results are qualitatively similar (fig. 8b), so only data from the biologically relevant green background was used for subsequent analysis.
Analysis of colour stimuli
As bees see colours differently to humans, I quantified stimuli according to five parameters: wavelength, brightness, purity (saturation), chromatic contrast to the background and green receptor contrast. Dominant wavelength was calculated by tracing a line from the centre of the colour hexagon through the stimulus location to the corresponding spectrum locus wavelength (Wyszecki and Stiles 1982). Brightness was measured as the sum of excitation values of the UV, blue and green receptors 本论文由英语论文网提供整理,提供论文代写,英语论文代写,代写论文,代写英语论文,代写留学生论文,代写英文论文,留学生论文代写相关核心关键词搜索。