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I analysed the most frequent flower colour according to a bees’ colour perception using the methods of Chittka, Shmida et al. (1994). I plotted the Australian flower colours in a colour space (Fig 5a). A colour space is a graphical representation of a bees’ colour perception. A radial grid of 10 degree sectors was placed over the distribution of colour loci and the number of floral colour loci within each sector was counted(fig. 5b).

Part 2. Does an Australian native bee (Trigona carbonaria) have innate colour preferences?

Insect model and housing

Trigona carbonaria is an Australian native stingless bee that lives in colonies of 4000-10000 individuals (Heard 1988). In the wild, stingless bees live in hollows inside trees (Dollin, Dollin et al. 1997). Trigona carbonaria has a similar social structure to the honeybee (Wille 1983). They are common to North Eastern Australia and are a potentially important pollinator for several major commercial crops (Heard 1999). A research colony (ca. 4000 adults and 800 foraging individuals) of T. carbonaria was propagated for the experiments by Dr Tim Heard (CSIRO Entomology, 120 Meiers Rd, Indooroopilly 4068, Australia) as described in the paper by Heard (1988). Bees were maintained in laboratory conditions so that no previous contact with flowers had been made. For this study, a colony was placed in a pine nest box (27.5 x 20 x 31 cm; LWH) and connected to the foraging arena by a 16 cm plexiglass tube, containing individual shutters to control bee movements. 

All laboratory experiments were conducted in a Controlled Temperature Laboratory (CTL) at Monash University, Clayton, School of Biological Sciences (CTL room G12C dimensions 3 x 5m), during the months of July 2009- January 2010. Relative humidity (RH) was set to 30%, and the temperature was set to 27 °C (SPER-Scientific Hygrometer, Arizona, USA), as this set up approximately matches conditions in Queensland for insect pollinators (Heard and Hendrikz 1993). Illumination (10/14 hr day/night) was provided by four Phillips Master TLS HE slimline 28W/865 UV+ daylight fluorescent tubes (Holland) with specially fitted high frequency (>1200Hz) ATEC Jupiter EGF PMD2x14-35 electronic dimmable ballasts which closely matches daylight conditions for trichromatic hymenoptera (Dyer and Chittka 2004). The flight arena (1.2 x 0.6 x 0.5m; LWH) was made of a coated steel frame with laminated white wooden side panels. The arena floor was painted foliage green, and the arena lid was covered with UV transparent plexiglass. Experiments were conducted from 1pm-3pm to control for time of day, as this is when bees are most active (Heard and Hendrikz 1993).

Pre-training

Bees were habituated to the flight arena for seven days. Naive foragers (i.e. bees that had never encountered real or artificial flowers) were initially pre-trained to forage in the flight arena on three rewarding aluminium sanded disks (25 mm in diameter), with a 10-μl droplet of 15% (w/w) sucrose solution placed in the centre. The disks were placed on vertical plastic cylinders (diameter = 25 mm, height = 100 mm), to raise them above the floor of the flight arena so that bees learnt to fly to the disks. Pre-training allows bees to become habituated to visiting artificial flowers for further experim本论文由英语论文网提供整理，提供论文代写，英语论文代写，代写论文，代写英语论文，代写留学生论文，代写英文论文，留学生论文代写相关核心关键词搜索。