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To estimate renal function in two different patients using creatinine clearance test.

Materials and method

Please refer to medical biochemistry practical book (BMS2).

Result:

The equation obtained from the graph used for the calculation of creatinine concentration is:

Y = 0.0008 X

Where: Y = absorbance

X = concentration of creatinine in umol/L.

P 1: X = 0.073/0.0008 = 91.25 umol/L

P 2: X = 0.346/0.0008 = 432 umol/L

U 1: X = 0.621/0.0008 = 776.25 x 21 = 16301.25 umol/L

U 2: X = 0.736/0.0008 = 468.75 x 21 = 9843.75 umol/L

Key:

P1 is plasma of patient 1.

P2 is plasma of patient 2.

U1 is urine sample of patient 1

U2 is urine sample of patient 2

Therefore the creatinine clearance is calculated by using the following formula:

(U/P) * (V/T) mls/min

U = urine creatinine umol/L

P = plasma creatinine umol/L

V = volume of urine passed in given time

T = time of collection

Creatinine clearance for patient 1:

= 16301.25/91.25 * 1000/1440

= 211.438 * 0.694

= 124 mls/min

Creatinine clearance for patient 2:

= 9843.75/ 432 * 3000/1440

= 22.786 * 2.083

= 47.46 mls/min

Conclusion:

From the above results I conclude that patient 1 has got normal renal function whereas patient 2 has renal failure.

Discussion

The creatinine calibration graph shows good linearity which indicates that the patient's results obtained are correct.

The clearance test more often concern about how much of a specific substance the kidney can remove from a given volume of blood in a stated period. In this experiment the creatinine clearance test was used to estimate GFR in two different patients based on the principle of Jaffe reaction (picric acid with alkaline solution from red to orange complex). A standard curve was produced using the absorbance reading against the standard concentration. A straight linear relationship obtained which means that as the optical density increase the creatinine concentration will increase. The equation obtained from the graph (Y = 0.0008 X) is used to calculate the plasma and urine creatinine concentration of two patients.

The plasma creatinine concentration for patient 1 is 91.25 umol/L, which means it is within the normal reference ranges (adult 60-120 umol/L).Marshal, (2000). Also the urine creatinine concentration (19293.75umol/L) for the same patient support the plasma concentration in which simply means that creatinine excretion by kidney was regularly. In contrast, patient 2 has got increased plasma creatinine concentration when compared with normal reference range which is 432 umol/L. That means the creatinine being not filtered regularly into urine.

There is no history of patient is provided and it is not stated whether the patient is male or female which can aid the diagnosis. However, there is not much difference between male and female normal reference ranges (adult 60-120 umol/L).

The clinical data are good in detec本论文由英语论文网提供整理，提供论文代写，英语论文代写，代写论文，代写英语论文，代写留学生论文，代写英文论文，留学生论文代写相关核心关键词搜索。